Effects of Cannabidiol Isolated or in Association With Risperidone in an Animal Model of Autism.

Researchers studied whether cannabidiol (CBD) could help with autism symptoms in rats. They gave pregnant rats a chemical that causes their babies to have autism-like behaviors, then treated the baby rats with CBD alone or CBD combined with risperidone (an autism medication). CBD by itself didn't help social behavior, but CBD combined with risperidone improved how well the rats interacted socially. This is early animal research, so more studies are needed before knowing if this applies to people.

This preclinical study investigated the effects of cannabidiol (CBD) alone and in combination with risperidone in a valproic acid-induced autism animal model. Pregnant rats were exposed to VPA to create offspring with autism-like behaviors including social deficits and developmental delays. The offspring were treated with CBD alone or CBD plus risperidone for 21 days. Results showed that while CBD alone did not improve social behavior, the combination of CBD with risperidone significantly enhanced sociability parameters.

The study also examined biochemical markers in brain tissue, finding changes in oxidative stress parameters. The findings suggest potential benefits of combining CBD with risperidone for autism-related symptoms, though this is preliminary animal research.

Results suggest CBD-risperidone combination therapy warrants investigation in human trials for autism social symptoms. However, this is preliminary animal research requiring significant additional study before clinical application. The lack of benefit from CBD alone challenges current clinical use patterns.

This is preclinical animal research using a chemical-induced autism model, which may not fully represent human autism. Sample size is not reported. The study duration was relatively short (21 days). Biochemical findings were mixed and difficult to interpret clinically.

Autism spectrum disorder (ASD) is characterized by deficits in communication, social interaction, and repetitive and stereotyped behaviors, with no specific drug therapy available. Studies have found that cannabidiol (CBD) can improve hyperactive and cognitive symptoms in children with ASD. However, little is known about the effect of CBD in combination with other medications, such as risperidone (RISP). This study aimed to evaluate the behavioral and biochemical effects of CBD in animals using a valproic acid (VPA)-induced ASD animal model.

VPA was administered in pregnant Wistar rats on Day 12.5 of gestation to induce the ASD model. From the 10th to the 16th postnatal day (PND), the neurodevelopment of the animals was assessed through eye-opening, olfactory discrimination, and negative geotaxis behavioral tests. From PNDs 9 to 54, the animals were weighed. They were treated for 21 days with CBD alone (100 mg/kg, by gavage, twice a day) or in combination with RISP (0.1 mg/kg, by gavage, once a day).

At PND 55, the animals were evaluated in social interaction and locomotor activity experiments. Finally, after behavioral assessment, the animals were euthanized, the brain was isolated, and oxidative stress parameters were evaluated in the hippocampus and cortex posterior. Animals exposed to VPA showed neurodevelopmental delays in opening their eyes, difficulties in turning around their axis, and took longer time to find the original nest when compared to control animals. They also exhibited impaired sociability and reduced exploratory activity, which indicates model impairments.

Interestingly, animals exposed to VPA treated with CBD + RISP significantly improved sociability parameters, whereas isolated CBD did not affect this parameter. In the biochemical analysis, a significant decrease in the hippocampal sulfhydryl content was noted in the CT + CBD group and an increase in the VPA + CBD group. In conclusion, these results suggest that CBD, in combination with RISP, may be an interesting pharmacological approach to reducing ASD-related symptoms. Summary: Besides the increased prevalence of ASD cases in recent years, there are no medications to improve the central symptoms of autism.

Numerous studies discuss CBD as an important medication for improving ASD symptoms; however, it is not known how CBD interacts with commonly used drugs in ASD individuals, such as RISP. This study demonstrated that CBD therapy, only when combined with RISP, improved sociability in a VPA-induced ASD animal model.